Platelet-derived growth factor suppresses and fibroblast growth factor enhances cytokine-induced production of nitric oxide by cultured smooth muscle cells. Effects on cell proliferation.

摘要: Stimulation of thymidine incorporation by basic fibroblast growth factor or epidermal treatment cultured quiescent smooth muscle cells (rat and human) was attenuated the concomitant with interleukin-1 beta in presence indomethacin. Platelet-derived factor-AB -BB-induced not inhibited cytokine under similar experimental conditions. Elevation nitrite levels conditioned medium cultures exposed to correlated inhibition incorporation. -BB production nitric oxide (measured as medium) treated simultaneously factor. However, platelet-derived factor-AA neither affected nor cells. Levels cytokine-stimulated were increased synergistically factors The elevation abolished nitro-L-arginine. Cultures maintained low for 9 days growth-inhibited, this reversed when culture supplemented cells, which grown coculture inserts induce production, before their combination other layers resulted second layer regardless used stimulation. Nitric may act an endogenous inhibitor cell proliferation vessel wall, impairment its be one action potent vascular mitogens such