Interaction between Schwann cells and osteoblasts in vitro.

摘要: Aim Given the well-known properties of Schwann cells in promoting nerve regeneration, transplanting into implant sockets might be an effective method to promote sensory responses osseointegrated implants. The aim this study was evaluate interaction between and osteoblasts. Methodology derived from sciatic nerves neonatal rat were co-culured with osteoblasts using Transwell inserts. proliferation co-culture system evaluated methylthiazol tetrazolium (MTT) colorimetric method. Moreover, secretions mRNA levels brain-derived neurotrophic factor (BDNF) growth (NGF) measured by enzyme-linked immunosorbent assay (ELISA) quantitative real-time PCR, respectively. In order test effect on osteoblasts, alkaline phosphatase (ALP) staining Alizerin red performed as well. Results cells, which co-cultured showed intact during observation period. gene expression synthesis BDNF NGF not impaired Meanwhile, exhibited a significant increase day 3 6 (P< 0.05). Co-culture these two types also led more intense ALP elevated number calcified nodules. Conclusion These findings demonstrate that, vitro indirect environment, can maintain their normal ability synthesize neurotrophins, then enhance differentiation