Toxicity and Mutagenicity of Patulin in Different Test Systems

摘要: Patulin, a mycotoxin produced by some Aspergillus, Penicillium, and Byssochlamys species, is found in rotten fruits fruit products. By its structure, an unsaturated α,β-lactone, it reacts with sulfhydryl groups. Patulin inhibits the growth rate of ciliate Tetrahymena at concentration 3.2 μg/ml 50%. Using same patulin concentration, protein synthesis this organism inhibited 85% RNA 86% compared control. Four hours after addition (3.2 μg/ml), DNA reduced to 20%; rises value control additional 2 h. An vitro system could be developed consisting permeabilized cells Tetrahymena. This allows separation regulatory secondary effects induced patulin. The reduces 50%, whereas are less than vivo system. A further characterization these results obtained experiments large fragment polymerase I. enzyme rather high concentrations.