Influence of nicotine on the biological activity of rabbit osteoblasts.
作者： Li Ma , Roger Arthur Zwahlen , Li Wu Zheng , Mai Har Sham
DOI: 10.1111/J.1600-0501.2010.02088.X
关键词:
摘要: Objectives: To assess the influence of nicotine on proliferation and gene expression osteogenic angiogenic mediators osteoblasts. Material methods: Rabbit primary osteoblasts were exposed to various concentrations (0.001, 0.1 10 μmol/l). The cell was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. transforming growth factor (TGF)-β1, bone morphogenetic protein (BMP)-2, platelet-derived (PDGF)-AA vascular endothelial (VEGF) evaluated using real-time reverse transcription – polymerase chain reaction. Results: osteoblast inhibited at concentration 0.001–10 μM 48 72 h culture, but with no significant effect 24 h. TGF-β1, BMP-2, PDGF-AA VEGF 10 μM, difference low 0.001 μM. Conclusions: Nicotine suppresses inhibits some key in vitro experimental model. These inhibitory effects activity may reflect, a certain degree, overall detrimental tobacco use survival rate dental implants. To cite this article: Ma L, Zwahlen RA, Zheng LW, Sham MH. Influence biological rabbit osteoblasts. Clin. Oral Impl. Res. 22, 2011; 338–342. doi: 10.1111/j.1600-0501.2010.02088.x
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Liran Levin, Devorah Schwartz-Arad, The effect of cigarette smoking on dental implants and related surgery. Implant Dentistry. ,vol. 14, pp. 357- 363 ,(2005) , 10.1097/01.ID.0000187956.59276.F8
Maria L. Pereira, Maria H. Fernandes, Fernando Peres, João C. Carvalho, Simultaneous effects of nicotine, acrolein, and acetaldehyde on osteogenic-induced bone marrow cells cultured on plasma-sprayed titanium implants. International Journal of Oral & Maxillofacial Implants. ,vol. 25, pp. 112- ,(2010)
Lavstedt S, Frithiof L, Bolin A, Eklund G, The effect of changed smoking habits on marginal alveolar bone loss. A longitudinal study. Swedish Dental Journal. ,vol. 17, pp. 211- ,(1993)
Z. Wang, W. Wu, X. Fang, Y. Wang, C. Xiao, R. Zhao, L. Wang, Z. Qiao, Protein expression changed by nicotine in rat vascular smooth muscle cells. Journal of Physiology and Biochemistry. ,vol. 63, pp. 161- 169 ,(2007) , 10.1007/BF03168227
OMER PELLETIER, Vitamin C status of cigarette smokers and nonsmokers. The American Journal of Clinical Nutrition. ,vol. 23, pp. 520- 524 ,(1970) , 10.1093/AJCN/23.5.520
Ken-ichi Nakahara, Hiroshi Nishimura, Makoto Kuro-o, Shun-ichi Takewaki, Misaki Iwase, Akiyuki Ohkubo, Yoshio Yazaki, Ryozo Nagai, Identification of three types of PDGF-A chain gene transcripts in rabbit vascular smooth muscle and their regulated expression during development and by angiotensin II. Biochemical and Biophysical Research Communications. ,vol. 184, pp. 811- 818 ,(1992) , 10.1016/0006-291X(92)90662-5
Li Ma, Li Wu Zheng, Mai Har Sham, Lim Kwong Cheung, Uncoupled angiogenesis and osteogenesis in nicotine-compromised bone healing Journal of Bone and Mineral Research. ,vol. 25, pp. 1305- 1313 ,(2010) , 10.1002/JBMR.19
Yasunari Kanda, Yasuhiro Watanabe, Nicotine-induced vascular endothelial growth factor release via the EGFR-ERK pathway in rat vascular smooth muscle cells. Life Sciences. ,vol. 80, pp. 1409- 1414 ,(2007) , 10.1016/J.LFS.2006.12.033
Russell L. Stedman, The chemical composition of tobacco and tobacco smoke. Chemical Reviews. ,vol. 68, pp. 153- 207 ,(1968) , 10.1021/CR60252A002
J.G. Meechan, I.D.M. Macgregor, S.N. Rogers, R.S. Hobson, J.P.C. Bate, M. Dennison, The effect of smoking on immediate post-extraction socket filling with blood and on the incidence of painful socket. British Journal of Oral & Maxillofacial Surgery. ,vol. 26, pp. 402- 409 ,(1988) , 10.1016/0266-4356(88)90093-9