Pathogenesis of Eastern Equine Encephalitis Virus in Mice and Development of a Second Generation Vaccine

摘要: Abstract : Eastern equine encephalitis virus (EEEV), an Alphavirus in the family Togaviridae, is important human and veterinary pathogen, considered most deadly of mosquito-borne alphaviruses due to high case fatality rate associated with clinical infections, reaching as 75% humans 90% horses. In patients that survive, neurologic sequelae are often devastating. Although natural infections acquired by mosquito bite, EEEV also highly infectious aerosol. This fact, along relative ease production stability this virus, has led it being identified a potential agent bioterrorism. Characterizing early events pathogenesis (FL93-939) various routes infection first step developing vaccine prevent disease. We hypothesize when mice challenged either intranasally or via aerosol will enter brain rapidly using olfactory system, which have implications for therapeutic development. The goal development produce product closely mimics infection; thereby stimulating appropriate effective immune response. However, new candidates should protect against both subcutaneous exposure virulent can be challenging. Formalin, INA, gamma irradiation been used inactivate viruses recently V3526. Inactivating attenuated-live provides additional layer safety formulation candidate. formalin, gamma-irradiation genetically modified strain (CVEV1219) but maintain its antigenic epitopes, creating valid candidate result protective responses parental (EEEVFL93-939).