Advances in instrumental methods for the measurement and speciation of trace metals.

摘要: Progress in understanding the role of trace metals biology has been largely dependent on development sensitive, accurate and precise analytical methods. Atomic spectroscopic techniques, particularly atomic absorption, have made greatest contribution. Key to success such techniques simplification sample processing so that contamination is minimized. Electrothermal atomization allowed sensitivity limits be lowered sufficiently even ultra-trace can detected. More recently, mass spectrometric detection metal ions added repertoire available instrumentation, with use inductively coupled plasma introduce into analyzer. These analyzers are suitable for multielement analysis. conventional analysis chelates offer an alternate solution but require considerable specimen preparation time. Intracellular localization necessitates complex prior instruments highly sophisticated expensive. Metal speciation a rapidly growing area research, major advances coming from coupling separation process, as capillary electrophoresis or high performance liquid chromatography, instrument detection. Inductively plasma-mass spectrometry proved excellent choice purposes. Refinement these methods well more widely microanalysis will add greatly continued our knowledge medicine.