Cloning and characterization of a novel cDNA that is IFN-gamma-induced in mouse peritoneal macrophages and encodes a putative GTP-binding protein.

摘要: Macrophage activation by IFN-gamma results in a cascade of gene expression. To identify genes activated mouse peritoneal macrophages IFN-gamma, we created cDNA subtraction library IFN-gamma-induced genes. We have isolated from this novel clone, called Mg21, whose mRNA is absent unstimulated and induced to high levels within 4 h following the addition IFN-gamma. Induction Mg21 occurred presence cycloheximide, indicating that expression does not require protein synthesis. A small amount was also LPS, but IL-2, IL-4, IL-10, or TNF-alpha. The DNA sequence 1617 nucleotides contains an open reading frame codes for 415 amino acids with predicted molecular weight 47,106 Da. acid lacks signal transmembrane segments, intracellular protein. Computer search GenBank EMBL databases indicates clone unique has 57% identity IRG-47, which pre-B B lymphocyte cell lines. IRG-47 encodes three conserved motifs present GTP-binding proteins. Analysis showed these are Mg21.