Impaired Macrophage Function Underscores Susceptibility to Salmonella in Mice Lacking Irgm1 (LRG-47)

摘要: IRG proteins, or immunity-related GTPases (also known as p47 GTPases), are a group of IFN-regulated proteins that highly expressed in response to infection. The localize intracellular membranes including vacuoles contain pathogens infected macrophages and other host cells. Current data indicate the protein Irgm1 (LRG-47) is critical for resistance bacteria. This function thought be consequence regulating survival vacuolar bacteria In current work, role controlling Salmonella typhimurium was explored further define mechanism through which regulates resistance. Irgm1-deficient mice displayed increased susceptibility this bacterium reflected bacterial loads spleen liver decreased maturation S. granulomas. also an inability concentrate at sites deposition. vitro, ability suppress growth impaired. Furthermore, adhesion motility after activation with IFN-γ markedly decreased. Altered adhesion/motility those cells accompanied by changes cell morphology, density adhesion-associated actin staining. Together, these suggest addition pathogen-containing vacuoles, plays key activated macrophages.