Diffraction-limited axial scanning in thick biological tissue employing an aberration correcting adaptive lens.

摘要: Diffraction-limited focusing deep into biological tissue is challenging due to spherical aberrations that lead a broadening of the focal spot particularly in axial direction. While diffraction-limit can be restored employing aberration correction with deformable mirror or spatial light modulator, bulky optical setup results required beam-folding. We propose bi actor adaptive lens, enables scanning and at same time specimen induced while offering compact setup. Using bi-actor lens confocal microscope, we show diffraction limited up \SI{340}{\micro m} inside phantom specimen. Applying this technique for in-vivo measurements zebrafish embryos reporter gene-driven fluorescence thyroid gland reveals substructures follicles.