The free nucleocapsids of the viral haemorrhagic septicaemia virus contain two antigenically related nucleoproteins.

作者: B. Basurco , F. Sanz , M. A. Marcotegui , J. M. Coll

DOI: 10.1007/BF01310666

关键词:

摘要: A protein of 34kDa (Nx) was induced in vitro by the infection fish cell cultures with rhabdovirus agent viral haemorrhagic septicaemia (VHS) trout. This only appeared as a major component concentrated or intracellular labeled VHS virus but not purified related infectious haematopoietic necrosis virus. That Nx is antigenically to nucleoprotein shown its reaction four antinucleoprotein monoclonal antibodies (at least 3 them reacting non competitivly against different epitopes) and immunoprecipitation polyvalent international reference sera. The be specifically associated free non-infective particles isolated ultracentrifugation which were confirmed nucleocapsids electron microscopy.

参考文章(20)
J. M. Coll, Addition of reducing agents to the peroxidase-o-phenylenediamine buffer reduces background of enzyme immunoassays. Revista española de fisiología. ,vol. 45, pp. 41- 46 ,(1989)
G Lenoir, P de Kinkelin, Fish rhabdoviruses: comparative study of protein structure. Journal of Virology. ,vol. 16, pp. 259- 262 ,(1975) , 10.1128/JVI.16.2.259-262.1975
P E McAllister, R R Wagner, Structural proteins of two salmonid rhabdoviruses. Journal of Virology. ,vol. 15, pp. 733- 738 ,(1975) , 10.1128/JVI.15.4.733-738.1975
Robert R. Wagner, Terry A. Schnaitman, Ruth M. Snyder, Structural Proteins of Vesicular Stomatitis Viruses Journal of Virology. ,vol. 3, pp. 395- 403 ,(1969) , 10.1128/JVI.3.4.395-403.1969
H C Ertl, B Dietzschold, M Gore, L Otvos, J K Larson, W H Wunner, H Koprowski, Induction of rabies virus-specific T-helper cells by synthetic peptides that carry dominant T-helper cell epitopes of the viral ribonucleoprotein. Journal of Virology. ,vol. 63, pp. 2885- 2892 ,(1989) , 10.1128/JVI.63.7.2885-2892.1989
Robert D. Gilmore, Jo-Ann C. Leong, The nucleocapsid gene of infectious hematopoietic necrosis virus, a fish rhabdovirus Virology. ,vol. 167, pp. 644- 648 ,(1988) , 10.1016/0042-6822(88)90130-4
Adolfo Z. Rueda, Julio M. Coll, Cloning of myelomas and hybridomas in fibrin clots. Journal of Immunological Methods. ,vol. 114, pp. 213- 217 ,(1988) , 10.1016/0022-1759(88)90176-7
N Lorenzen, NJ Olesen, PE Vestergard Jorgensen, Production and characterization of monoclonal antibodies to four Egtved virus structural proteins. Diseases of Aquatic Organisms. ,vol. 4, pp. 35- 42 ,(1988) , 10.3354/DAO004035
Bernhard Dietzschold, Monique Lafon, Honghai Wang, Laszlo Otvos, Esteban Celis, William H. Wunner, Hilary Koprowski, Localization and immunological characterization of antigenic domains of the rabies virus internal N and NS proteins. Virus Research. ,vol. 8, pp. 103- 125 ,(1987) , 10.1016/0168-1702(87)90023-2