A new enzymatic method for the determination of glycollate in urine and plasma
作者： G.P. Kasidas , G.Alan Rose
DOI: 10.1016/0009-8981(79)90048-2
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摘要: An enzymatic assay for the measurement of glycollate in urine and plasma is described. Gycollic acid oxidase (glycollate:oxygen oxidoreductase, EC 1.1.3), extracted from spinach leaves, used oxidation to produce glyoxylate hydrogen peroxide. In presence peroxidase, peroxide oxidatively couples sulphonated 2,4-dichlorophenol 4-aminophenazone form a soluble purple quinoneimine dye. Interfering substances are removed by deproteinisation adsorption onto activated charcoal before analysis. Glycollic also catalyses lactic which therefore has be determined separately. The mean normal urinary adults 0.19 mmol/24 h with no difference between males females. 0.17 mmol/l, but higher than
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