In vitro propagation and cloning of murine natural suppressor (NS) cells.

摘要: During a short period of time after birth or radiotherapy, the spleens neonatal and adult TLI-treated mice contain suppressor cells mixed leukocyte reaction (MLR) graft-vs-host disease. The present report shows that MLR suppressive activity spleen from BALB/c can be maintained in long-term tissue culture by using conditioned medium. cloned limiting dilution, reproducibly inhibit [3H]TdR incorporation at responder-to-suppressor cell ratios 50:1. There is no antigen specificity H-2 haplo-type restriction suppression. do not per se, because inhibition was observed co-culture experiments with EL4 tumor line IL 2-dependent HT-2 line. By immunofluorescent staining techniques, surface phenotype found to similar reported previously for NK (Thy-1+, Lyt-1-, Lyt-2-, Ig-, Ia-, MAC-1-, asialo-GM1+). However, lines showed natural killer when YAC-1 target were used. Thus, have been termed "natural suppressor" indicate marker similarities cells, both vivo vitro, but different effector functions.