Functional Aspects of Chromosome Organization: Scaffold Attachment Regions and their Ligands

摘要: Publisher Summary This chapter discusses the nature of DNA scaffold interaction, identifies proteins involved in this binding, and analyzes role attached regions (SAR)-mediated loop or domain organization replication transcriptional control. The scaffold-attachment assay all sequences with potential to bind scaffold. affinity SARs for appears be maintained metaphase. In Drosophila, SAR fragments identified by association interphase were mapped metaphase scaffolds, both from Drosophila Hela cells. No major differences binding specificity are detected among seven tested Drosophila. more surprising aspects is cross-species' conservation binding. -derived have been shown human, rat, mouse, yeast scaffolds. various other sources show similar abilities heterologous view lack a strictly defined consensus sequence, it seems likely that conserved conformation defines properties attachment region. Studies lithium 3’5’-diiodosalicylate (LIS)-extracted scaffolds budding fission origins (ARS elements) specifically associated nuclear Despite difficulty identifying higher eukaryotic cells, there also suggestive evidence located 5‘ chick α-globin gene 3’ Chinese hamster dehydrofolate reductase close sites.