Poly(glycidyl methacrylate-co-3-thienyl-methylmethacrylate) based working electrodes for hydrogen peroxide biosensing
作者： Seyda Korkut Ozoner
DOI: 10.1002/JCTB.2695
关键词:
摘要: BACKGROUND: Hydrogen peroxide biosensors based on Poly(glycidyl methacrylate-co-3-thienylmethylmethacrylate)/ Polypyrrole [Poly(GMA-co-MTM)/PPy] composite film were reported. Poly(GMA-co-MTM) including various amounts of GMA and MTM monomers was synthesized via the radical polymerization. Enzyme horseradish peroxidase (HRP) trapped in Poly(GMA-co-MTM)/PPy composites during electropolymerization reaction between pyrrole thiophene groups monomer, chemically bonded epoxy GMA. Analytical parameters fabricated electrodes calculated are discussed terms electroactivity mass transfer conditions films. RESULTS: The amount electroactive HRP found to be 1.25, 0.34 0.213 µg for working Poly(GMA30%- co-MTM70%)/PPy/HRP, Poly(GMA85%-co-MTM15%)/PPy/HRP Poly(GMA90%-co-MTM10%)/PPy/HRP, respectively. Optimal response obtained at pH 7 an operational potential − 0.35 V. It observed that effective enzyme immobilization films could changed by changing ratios fractions electrodes. CONCLUSION: increases with increasing content final copolymer. High stabilities can attributed strong covalent linkage due preventing deterioration loss. A more convenient microenvironment provided higher ratios. is dominated mechanism electron obtain sensitivity values. This work contributes discussions clarifying problems regarding design biosensors. Copyright © 2011 Society Chemical Industry
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