[15] Radioiodination of cell surface lipids and proteins for use in immunological studies

摘要: Publisher Summary This chapter describes the use of radioiodinated diazotized iodosulfanilic acid (ISA) and a lactoperoxidase-catalyzed iodination method for labeling examination plasma membrane proteins lipids in cells under humoral immune attack. There are currently available several reagents, including ISA NaI-lactoperoxidase (LPO) complex, that have been used to label cell membranes radioisotopically, presumably by exposed tyrosine and/or histidine residues on proteins. The NaI reaction has shown variety surface lipids, phospholipids, triacylglyceddes, free fatty acids, lysophosphatides. is enzyme-dependent its initial steps, which an oxidizing agent (H 2 O ) generated, with subsequent oxidation I – (from NaI) . final interaction I2, lipid molecules, does not involve simple addition unsaturated constituents because fully saturated acids or acid-containing neutral phospholipids can also be labeled this reaction. suggests substitution exchange reactions occur system.