Physical and Chemical Composition of Subcellular Fractions from Tumor Cells Treated with Metabolic Inhibitors or Hormones

摘要: Line 10 guinea pig hepatoma cells are resistant to killing by antibody plus complement but not human complement. Agents that increase (metabolic inhibitors) or decrease (hormones) the sensitivity of were examined for their effects on chemical, physical, and enzymic composition cells. The these agents chemical characteristics isolated plasma membrane intracellular fractions also measured. Adriamycin treatment resulted in a amount ribonucleoprotein smooth endoplasmic reticulum from as compared untreated Hormone (insulin hydrocortisone)-treated enhanced yield this fraction decreased mitochondria controls. Generally, drug-treated decreased, whereas hormone-treated enhanced, protein, lipid phosphate, total phosphate content, This pattern was noted and, several cases, fractions. These data suggest content membranes may correlate with ability resist humoral immune attack.