Detection and Partial Characterization of Human T and B Lymphocyte Membrane Antigens with Antisera to HSB and SB Cell Lines

摘要: Antisera were produced in rabbits and monkeys by immunization with either HSB (T cell) or SB (B lymphoblastoid cell lines. With a C-dependent microcytotoxicity assay, appropriately absorbed antisera to strongly reactive human thymocytes, T lines, some acute lymphoblastic leukemia cells (>90% lysis). Approximately 75% of normal peripheral blood lymphocytes (PBL) lysed the antisera. Reactivity was at background level when monocytes from patients chronic lymphocytic (CLL), myelogenous (CML), most myelocytic (AML) myelomonocytic (AMML) tested against Further absorption anti-HSB sera CML platelets did not remove serologic reactivity, whereas thymus, PBL, concanavalin A- (Con A) stimulated PBL removed reactivity all tested. B CLL cells, tonsil, adenoid, enriched preparations AML AMML patients. Additional anti-SB reactivity. Anti-SB that additionally negative Sodium deoxycholate- (DOC) solubilized membrane variety labeled 125 I subjected radioimmunoprecipitation (RIP) polyacrylamide gel electrophoresis (PAGE) techniques. Unlabeled DOC-solubilized antigens able inhibit cytotoxic reaction thymocytes. The target affected antigens. DOC HSB, thymocytes block RIP activity sera. One major peak radioactivity (m.w. approximately 150,000 daltons) consistently observed during PAGE reduced immunoprecipitates monkey rabbit surface thymus. Treatment additional 2-mercaptoethanol reduction dithiothreitol failed alter m.w. antigen. No significant peaks same precipitated SB. membranes revealed two radioactivity, corresponding 38,000 30,000 daltons. When SB, K562, no observed.