Development and evaluation of a self-cleaning custom-built auto sampler controlled by a low-cost RaspberryPi microcomputer for online enzymatic activity measurements

摘要: A fully automated on-site device (SAMP-FIL) that enables water sampling with simultaneous filtration and effective cleaning procedures of the device's components was developed field-tested. The SAMP-FIL custom-built using commercially available controlled by a RaspberryPi single-board computer operating open-source software. designed for sample pre-treatment minimal alteration to meet requirements measurement devices cannot handle coarse suspended solids within procedure or cycle. highly provides fresh minimally altered connected device. construction programmed software facilitates use different devices. pretreatment tested over one year rapid enzymatic activity (beta-d-glucuronidase, GLUC) determination (BACTcontrol) in sediment-laden stream water. formerly used proprietary set-up assumed lead significant damping signal due its susceptibility clogging, debris accumulation bio-film accumulation. implementation considerably increased error-free running time accuracy BACTcontrol This paper describes how low-cost microcomputers, such as RaspberryPi, can be operators substantially improve established measuring systems via Furthermore, results this study highlight importance adequate quality measurements.