Oscillatory dynamics of cell cycle proteins in single yeast cells analyzed by imaging cytometry.

摘要: Progression through the cell division cycle is orchestrated by a complex network of interacting genes and proteins. Some these proteins are known to fluctuate periodically during cycle, but systematic study fluctuations broad sample cell-cycle has not been made until now. Using time-lapse fluorescence microscopy, we profiled 16 strains budding yeast, each containing GFP fused single gene involved in regulation. The dynamics protein abundance localization were characterized extracting amplitude, period, other indicators from series images. Oscillations could clearly be identified for Cdc15, Clb2, Cln1, Cln2, Mcm1, Net1, Sic1, Whi5. period oscillation fluorescently tagged generally good agreement with inter-bud time. very strong oscillations Net1 Mcm1 expression remarkable since little about temporal genes. By collecting data large samples cells, quantified some aspects cell-to-cell variability due presumably intrinsic extrinsic noise affecting cycle.