Identification of incompatibility genotypes in almond (Prunus dulcis Mill.) using specific primers based on the introns of the S-alleles

摘要: Identification of the incompatibility genotypes almond cultivars is important in breeding programmes for designing crosses and selecting progeny. This paper describes a novel molecular technique identification S-alleles based on use PCR primers designed from sequences introns without need restriction enzyme digestion. Nine specific pairs have been S1, S2, S5, S7, S8, S9, S10 (putative), S23 Sf alleles, these confirmed S-allele specificities 22 23 accessions which published information available. provides precise method identifying genomic DNAs cultivars, will be useful confirming segregation alleles