Novel genotypes in Helicobacter pylori involving domain V of the 23S rRNA gene.

摘要: Background: Helicobacter pylori is a pathogenic bacterium that infects half of the human population. In Chile, between 55% and 79% people are colonized by H. . At present, therapeutic strategies to eradicate depend on knowledge its resistance antibiotics. The clarithromycin in associated with point mutations 23S rRNA. This study analyzes rRNA gene minimum inhibitory concentration (MIC) for isolates from patients metropolitan region Chile. Materials methods: 50 dyspeptic no history exposure were tested agar dilution method. Resistant strains analyzed polymerase chain reaction-based restriction fragment length polymorphism sequencing. Results: Primary was observed 10 (20%). A single mutation detected four two or more other six cases. C2147G transversion G1939A, T1942C, A2142G transitions peptidyltransferase domain V novel. Conclusions: shows: 1, novel variants gene; 2, high prevalence displaying primary low level MIC an urban area Metropolitan Region