Production of phoA promoter-controlled human epidermal growth factor in fed-batch cultures of Escherichia coli YK537 (pAET-8)

摘要: Abstract Escherichia coli YK537 (pAET-8) was used to produce human epidermal growth factor (hEGF), which expressed under the control of phoA promoter and secreted culture medium. In batch using a complex medium, phosphate nearly depleted at 8 h, but subsequently accumulated reached 3.8 mM. The high concentration resulted in low hEGF expression level 28 mg l−1. Hydrolysis organophosphorus medium by extracellular enzymes caused accumulation phosphate, supply glucose led consumption released phosphate. Feeding pH-stat strategy effective maintaining levels, avoided acetic acid. production 281 mg l−1. When fed with essential amino acids leucine proline, recombinant 332 mg l−1.