On the histogenesis of Ewing's sarcoma: An ultrastructural, immunohistochemical, and cytochemical study

摘要: Forty-two cases of Ewing's sarcoma (ES) have been studied with light microscopy during the 9-year period 1974 to 1982. Thirty-three patients had ES bone, and in 9 tumor was located extraskeletal soft tissues. Cases which fulfilled all morphologic criteria were accepted as typical (31 cases), those some architectural or cytologic peculiarities considered atypical forms (11 cases). An immunohistochemical study (PAP method) evaluate presence cells following markers: myoglobin, F-VIII-related antigen, lysozyme, alpha-1-antitrypsin, alpha-1-antichymotrypsin, immunoglobulins (IgG, IgA, IgM, kappa lambda chains), performed negative results (paraffin blocks available 38 The cytochemical on fresh tissue imprints from five (PAS, Sudan Black, alpha-naphthyl acetate esterase, acid phosphatase, beta glucuronidase, myeloperoxidases, naphthol-AS-D chloroacetate esterase alkaline phosphatase) gave no pattern histogenetic significance, PAS being best marker sections touch preparations. A detailed ultrastructural 34 cases; main findings may be summarized follows: medium sized cells, polygonal shape, oval nuclei, smooth nuclear envelope, abundant euchromatin, well-developed nucleolonema, scant membranous organelles, hyaloplasmic glycogen, occasional lipid vacuoles, straight cell membranes, primitive intercellular junctions. No differences found between bone ES; moreover, showed moderate quantitative qualitative change. histogenesis is discussed; functional markers suggest origin; however, lines excluded. It impression authors that they are dealing a noncommitted mesenchymal cell.