Rapid clonal propagation of guava through in vitro shoot proliferation on nodal explants of mature trees

摘要: In vitro clonal propagation of guava ‘Banaras local’ was achieved by culturing nodal explants mature trees on Murashige and Skoog (MS) revised medium supplemented with 4.5 μM 6-benzyladanine (BA) alone or in combination either 0.6 indole-3-acetic acid (IAA), 0.5 indole-3-butyric (IBA) 0.3 gibberellic (GA3). Multiple shoots were induced to form enhancement axillary branching BA (4.5 μM) without any auxin gibberellin found give best shoot multiplication rate. this 3–6 developed collected from field-grown plants 5–10 taken proliferated within 12 wk culture. A prior transfer clumps a containing lower concentration (0.5 before harvesting cuttings for rooting allowed rapid extension growth increased the number usable per Adventitious occurred after subculturing excised 1/2 strength MS salts, 1.5% sucrose, 1 each IBA α-naphtha-leneacetic (NAA), gl-1 activated charcoal. Regenerated plantlets successfully established soil.