Sequence and expression analysis of bovine pigment epithelium-derived factor

摘要: PEDF, a member of the serpin superfamily proteins related through their highly conserved folded conformation, has neurotrophic properties, including promotion neurite-outgrowth and neuronal survival. Previously, we have purified characterized PEDF protein from extracellular matrixes bovine eyes. Here, show cDNA sequence expression analysis PEDF. Northern RNA retinal pigment epithelium (RPE) neural retina using human fragment reveals gene only for RPE. Sequence clone isolated RPE predicts polypeptide 416 amino acid residues that shares 88.6% 85% identity with mouse respectively. It an N-terminal signal peptide, consensus glycosylation site homology serpins required maintaining tertiary structure. Cell-free by in vitro transcription translation yields precursor 45,000-Mr immunoprecipitates antibody to Expression stably transfected baby hamster kidney cells shows recombinant is secreted culture media as mature 50,000-Mr protein, which induces on retinoblastoma cells, like naturally-occurring protein. Thus, here codes functional soluble glycoprotein.