Biodegradation of Butachlor by Rhodococcus sp. Strain B1 and Purification of Its Hydrolase (ChlH) Responsible for N-Dealkylation of Chloroacetamide Herbicides
作者： Hong-Ming Liu , Li Cao , Peng Lu , Haiyan Ni , Yun-Xiang Li
DOI: 10.1021/JF303936J
关键词:
摘要: Rhodococcus sp. strain B1 could degrade 100 mg/L butachlor within 5 days. Butachlor was first hydrolyzed by through N-dealkylation, which resulted in the production of butoxymethanol and 2-chloro-N-(2,6-dimethylphenyl)acetamide. Butoxymethanol be further degraded utilized as carbon source for growth B1, whereas 2-chloro-N-(2,6-dimethylphenyl)acetamide not further. The hydrolase designated ChlH, responsible N-dealkylation side chain butachlor, purified 185.1-fold to homogeneity with 16.1% recovery. optimal pH temperature ChlH were observed 7.0–7.5 30 °C, respectively. This enzyme also able catalyze other chloroacetamide herbicides; catalytic efficiency followed order alachlor > acetochlor >butachlor pretilachlor, indicated that alkyl length influenced herbicides. is report on biodegradation c...
acs.org
sci-hub.se 参考文章(40)
S Chapalmadugu, G R Chaudhry, Isolation of a constitutively expressed enzyme for hydrolysis of carbaryl in Pseudomonas aeruginosa. Journal of Bacteriology. ,vol. 175, pp. 6711- 6716 ,(1993) , 10.1128/JB.175.20.6711-6716.1993
J F Mayaux, E Cerbelaud, F Soubrier, P Yeh, F Blanche, D Pétré, Purification, cloning, and primary structure of a new enantiomer-selective amidase from a Rhodococcus strain: structural evidence for a conserved genetic coupling with nitrile hydratase. Journal of Bacteriology. ,vol. 173, pp. 6694- 6704 ,(1991) , 10.1128/JB.173.21.6694-6704.1991
R Behki, E Topp, W Dick, P Germon, Metabolism of the herbicide atrazine by Rhodococcus strains. Applied and Environmental Microbiology. ,vol. 59, pp. 1955- 1959 ,(1993) , 10.1128/AEM.59.6.1955-1959.1993
W W Mulbry, J S Karns, P C Kearney, J O Nelson, C S McDaniel, J R Wild, Identification of a plasmid-borne parathion hydrolase gene from Flavobacterium sp. by southern hybridization with opd from Pseudomonas diminuta. Applied and Environmental Microbiology. ,vol. 51, pp. 926- 930 ,(1986) , 10.1128/AEM.51.5.926-930.1986
S. Dwivedi, B.R. Singh, A.A. Al-Khedhairy, S. Alarifi, J. Musarrat, Isolation and characterization of butachlor-catabolizing bacterial strain Stenotrophomonas acidaminiphila JS-1 from soil and assessment of its biodegradation potential. Letters in Applied Microbiology. ,vol. 51, pp. 54- 60 ,(2010) , 10.1111/J.1472-765X.2010.02854.X
S A Denome, C Oldfield, L J Nash, K D Young, Characterization of the desulfurization genes from Rhodococcus sp. strain IGTS8. Journal of Bacteriology. ,vol. 176, pp. 6707- 6716 ,(1994) , 10.1128/JB.176.21.6707-6716.1994
H.-C. Chiang, J.-R. Duh, Y.-S. Wang, Butachlor, thiobencarb, and chlomethoxyfen movement in subtropical soils. Bulletin of Environmental Contamination and Toxicology. ,vol. 66, pp. 1- 8 ,(2001) , 10.1007/S0012800197
Weiliang Shen, Honghong Chen, Kaizhi Jia, Jun Ni, Xin Yan, Shunpeng Li, Cloning and characterization of a novel amidase from Paracoccus sp. M-1, showing aryl acylamidase and acyl transferase activities Applied Microbiology and Biotechnology. ,vol. 94, pp. 1007- 1018 ,(2012) , 10.1007/S00253-011-3704-6
Zhichun Wang, Yangyang Wang, Fenfen Gong, Juan Zhang, Qing Hong, Shunpeng Li, Biodegradation of carbendazim by a novel actinobacterium Rhodococcus jialingiae djl-6-2 Chemosphere. ,vol. 81, pp. 639- 644 ,(2010) , 10.1016/J.CHEMOSPHERE.2010.08.040
Bao-Jian Hang, Qing Hong, Xiang-Ting Xie, Xing Huang, Cheng-Hong Wang, Jian He, Shun-Peng Li, SulE, a Sulfonylurea Herbicide De-Esterification Esterase from Hansschlegelia zhihuaiae S113 Applied and Environmental Microbiology. ,vol. 78, pp. 1962- 1968 ,(2012) , 10.1128/AEM.07440-11