A Novel Aldo-Keto Reductase (AKR17A1) of Anabaena sp. PCC 7120 Degrades the Rice Field Herbicide Butachlor and Confers Tolerance to Abiotic Stresses in E. coli.
作者： Chhavi Agrawal , Sonia Sen , Shivam Yadav , Shweta Rai , Lal Chand Rai
DOI: 10.1371/JOURNAL.PONE.0137744
关键词:
摘要: Present study deals with the identification of a novel aldo/keto reductase, AKR17A1 from Anabaena sp. PCC7120 and adds on as 17th family AKR superfamily drawn wide variety organisms. shares many characteristics typical such as— (i) conferring tolerance to multiple stresses like heat, UV-B, cadmium, (ii) excellent activity towards known substrates (isatin 2-nitrobenzaldehyde), (iii) obligate dependence NADPH cofactor for enzyme activity. The most attribute AKR17A1, first reported in this study, is its capability metabolize butachlor, persistent rice field herbicide that adversely affects agro-ecosystem non-target catalyzed- degradation butachlor resulted into formation 1,2-benzene dicarboxylic acid 2,6 bis (1,1, dimethylethyl) 4,-methyl phenol major products confirmed by GC-MS analysis.
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