Phorbol ester-induced myeloid differentiation is mediated by protein kinase C-alpha and -delta and not by protein kinase C-beta II, -epsilon, -zeta, and -eta.

摘要: It is generally accepted that the multiple, similar protein kinase C (PKC) isozymes are responsible for different specialized physiological processes, but evidence directly assigns specific functions to scarce. To test whether PKC involved in myeloid differentiation, we have studied effect of overexpression PKC-alpha, -beta II, -delta, -epsilon, -zeta and -eta 32D, a mouse progenitor cell line does not differentiate response 12-O-tetradecanoylphorbol-13-acetate (TPA). No significant morphological or phenotypic changes could be observed unstimulated cells overexpress any these isozymes. However, lines overexpressed PKC-alpha -delta had acquired ability become mature macrophages 2-6 h after TPA stimulation. The PKC-beta -zeta, -eta, contrast, did permit TPA-induced differentiation. These results indicate only two members gene family can participate