Constitutive signaling activity of a receptor-associated protein links fertilization with embryonic patterning in Arabidopsis thaliana.

作者: Ancilla Neu , Emily Eilbert , Lisa Y. Asseck , Daniel Slane , Agnes Henschen

DOI: 10.1073/PNAS.1815866116

关键词:

摘要: In flowering plants, the asymmetrical division of zygote is first hallmark apical-basal polarity embryo and controlled by a MAP kinase pathway that includes MAPKKK YODA (YDA). Arabidopsis, YDA activated membrane-associated pseudokinase SHORT SUSPENSOR (SSP) through an unusual parent-of-origin effect: SSP transcripts accumulate specifically in sperm cells but are translationally silent. Only after fertilization protein transiently produced zygote, presumably from paternally inherited transcripts. recently diverged, Brassicaceae-specific member BRASSINOSTEROID SIGNALING KINASE (BSK) family. BSK proteins typically play broadly overlapping roles as receptor-associated signaling partners various receptor pathways involved growth innate immunity. This raises two questions: How did with generic function signal relay acquire property signal-like patterning cue, how early process plants outside Brassicaceae family, where orthologs absent? Here, we show Arabidopsis BSK1 BSK2, close paralogs conserved several YDA-dependent events, including embryogenesis. However, contribution to activation does not overlap contributions BSK2. The loss intramolecular regulatory interaction enables constitutively activate pathway, thus initiates soon translated without necessity invoking canonical activation.

参考文章(58)
Wolfgang Lukowitz, Adrienne Roeder, Dana Parmenter, Chris Somerville, A MAPKK Kinase Gene Regulates Extra-Embryonic Cell Fate in Arabidopsis Cell. ,vol. 116, pp. 109- 119 ,(2004) , 10.1016/S0092-8674(03)01067-5
Michaela Spitzer, Jan Wildenhain, Juri Rappsilber, Mike Tyers, BoxPlotR: a web tool for generation of box plots Nature Methods. ,vol. 11, pp. 121- 122 ,(2014) , 10.1038/NMETH.2811
Hua Shi, Haojie Yan, Juan Li, Dingzhong Tang, BSK1, a receptor-like cytoplasmic kinase, involved in both BR signaling and innate immunity in Arabidopsis Plant Signaling & Behavior. ,vol. 8, ,(2013) , 10.4161/PSB.24996
Shivakumar Sreeramulu, Yana Mostizky, Sukumaran Sunitha, Eilon Shani, Hadas Nahum, Dor Salomon, Liat Ben Hayun, Christian Gruetter, Daniel Rauh, Naomi Ori, Guido Sessa, BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis. Plant Journal. ,vol. 74, pp. 905- 919 ,(2013) , 10.1111/TPJ.12175
Xiangzong Meng, Huachun Wang, Yunxia He, Yidong Liu, John C. Walker, Keiko U. Torii, Shuqun Zhang, A MAPK Cascade Downstream of ERECTA Receptor-Like Protein Kinase Regulates Arabidopsis Inflorescence Architecture by Promoting Localized Cell Proliferation The Plant Cell. ,vol. 24, pp. 4948- 4960 ,(2013) , 10.1105/TPC.112.104695
José M Alonso, Anna N Stepanova, Thomas J Leisse, Christopher J Kim, Huaming Chen, Paul Shinn, Denise K Stevenson, Justin Zimmerman, Pascual Barajas, Rosa Cheuk, Carmelita Gadrinab, Collen Heller, Albert Jeske, Eric Koesema, Cristina C Meyers, Holly Parker, Lance Prednis, Yasser Ansari, Nathan Choy, Hashim Deen, Michael Geralt, Nisha Hazari, Emily Hom, Meagan Karnes, Celene Mulholland, Ral Ndubaku, Ian Schmidt, Plinio Guzman, Laura Aguilar-Henonin, Markus Schmid, Detlef Weigel, David E Carter, Trudy Marchand, Eddy Risseeuw, Debra Brogden, Albana Zeko, William L Crosby, Charles C Berry, Joseph R Ecker, Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana Science. ,vol. 301, pp. 653- 657 ,(2003) , 10.1126/SCIENCE.1086391
Elena D. Shpak, Diverse roles of ERECTA family genes in plant development. Journal of Integrative Plant Biology. ,vol. 55, pp. 1238- 1250 ,(2013) , 10.1111/JIPB.12108
Caroline A Schneider, Wayne S Rasband, Kevin W Eliceiri, NIH Image to ImageJ: 25 years of image analysis Nature Methods. ,vol. 9, pp. 671- 675 ,(2012) , 10.1038/NMETH.2089