Up-regulation of urokinase and urokinase receptor genes in malignant astrocytoma.
作者： Candece L Gladson , Mitchell Alan Olman , I. Z. Yacoub , V. Pijuan-Thompson , G. Y. Gillespie
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摘要: Abstract To understand the role of urokinase (u-PA) and receptor (u-PAR) in malignant astrocytoma cell invasion normal brain, astrocytic expression u-PAR u-PA mRNAs were analyzed by riboprobe situ hybridization non-neoplastic brain biopsies. In eight astrocytomas (glioblastomas), mRNA was demonstrated, whereas seven biopsies, not expressed. one four low grade all anaplastic expressed, although undetectable. Consistent with detection, proteins expressed astrocytes five glioblastoma To study tumor margin, U-251MG cells propagated intracerebrally a severe combined immunodeficient mouse xenograft (28 days), found to localize predominantly leading edge, throughout tumor. Furthermore, adherent human vitro proteins, which localized sites integrin alpha nu beta 3 cell-matrix contacts. These data indicate that co-expression marks astrocyte phenotype bound may play virtue its at edge.
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