Modulation of in Vitro Invasion of Human Glioblastoma Cells by Urokinase-type Plasminogen Activator Receptor Antibody

摘要: Four human glioblastoma cell lines (U251, UWR1, UWR2, and UWR3) were tested for the expression of surface receptor urokinase-type plasminogen activator (uPA). To our knowledge there have been no previous reports about uPA receptors (uPARs) in lines. All four we found to bind recombinant Pro-uPA saturably reversibly. Scatchard analysis radioligand binding with acid-pretreated cells showed presence a single population high-affinity uPARs on cells. Northern blot confirmed that like other express 1.4-kilobase uPAR mRNA 2.4-kilobase mRNA. The significance invasive potential was examined by incubating antibody an vitro invasion assay. anti-uPAR monoclonal blocked effectively Matrigel assay, which inhibition ranged between 20 57% studied. These data suggest contribute significantly capacity cells, possibly facilitating activity.