The bleb formation of the extracellular pseudopodia; early evidence of microtubule depolymerization by estramustine phosphate in glioma cell; in vitro study.

摘要: Estramustine phosphate (EMP) is an anti-microtubule agent that depolymerizes microtubules and also causes apoptosis of glioma cells. Both these pharmacological actions have been previously studied within the same cytotoxic range EMP concentrations. The purpose this study was to investigate which two phenomena occurred before other. A preliminary MTT assay done distinguish non-cytotoxic (0.005–0.1 μM) (0.5–10 for BT4C To apoptotic changes, transmission electron microscopy (TEM), DNA laddering, in situ endo-labeling (TUNEL) method were employed. chemotaxis used assess cell motility. Scanning TEM immunocytochemistry with anti-β tubulin antibody applied detect morphological changes microtubules. Suppression motility by doses group attributed cyto-reductive effect, relating apoptosis. At 0.01–0.1 μM (non-cytotoxic doses), did not indue concentrations, immunohistochemistry revealed formation blebs on tip pseudopodia contained abnormally depolymerized microtubules, a finding observed at low temperature or during migration. Cell significantly inhibited cytostatic (0.05 0.1 μM). Bleb might be evidence abnormal disassembly prior induction In cells probably initiates causing depolymerization Inhibition could beneficial support other therapies.