Intracellular accumulation and cytotoxicity of doxorubicin with different pharmaceutical formulations in human cancer cell lines

摘要: The structure of both carrier and anticancer drug affects the intracellular fate a transported drug. study investigated in vitro accumulation cytotoxic activity doxorubicin-loaded solid lipid nanoparticles (SLN), doxorubicin pegylated liposomes (Caelyx®) free doxorubicin. Intracellular levels were determined by high performance liquid chromatography with fluorescence detection, trypan blue dye exclusion assay, respectively. Doxorubicin-loaded SLN inhibited cell growth more strongly than either or liposomal doxorubicin, human colorectal adenocarcinoma, HT-29, retinoblastoma Y79, glioblastoma U373 lines. IC 50 values for significantly lower after 24 h exposure those all lines; 48 they HT-29 Y79 cells. enhanced was associated increased incorporation cells: to drug-loaded versus Rate also differed among different particular, cells epithelial origin found be sensitive SLN. In conclusion, greater sensitivity other formulations may due capability delivery system enhance action, through marked uptake within cell.