Rifaximin anti-inflammatory activity on bovine endometrium primary cell cultures: a preliminary study
作者： Lisa Flammini , Leonardo Mantelli , Alessandra Volpe , Giuseppe Domenichini , Rosanna Di Lecce
DOI: 10.1002/VMS3.115
关键词:
摘要: Rifaximin is an unabsorbed oral antibiotic showing anti-inflammatory properties in human pathologies like irritable bowel syndrome and inflammatory disease. In veterinary medicine, rifaximin primarily used the treatment of dermatological diseases all animal species, therapy prophylaxis mastitis cows endometritis cattle horses. The aim this preliminary study was to evaluate on primary cell cultures from bovine endometrium which response induced by Lipopolysaccaride (LPS) treatment. Epithelial stromal cells were isolated separately incubated for 24 h with 1 μg mL-1 LPS after (10, 50 100 μmol L-1 ) or dexamethasone (10 pre-treatment h. Supernatants collected interleukin (IL)-6 IL-8 accumulation measured ELISA. dose dependently inhibited LPS-induced increase IL-6 cells, whereas epithelial it not possible detect any these interleukins. reduced production, a potential effect that opens up new possibilities use drug uterine diseases.
sci-hub.se 参考文章(17)
M. Masi, J. Paggi, S. Belluzzi, B. Truszkowska, Behaviour of rifaximin in foam formulation after intrauterine administration. Summa. ,vol. 7, pp. 98- 102 ,(1990)
Andrea Mencarelli, Barbara Renga, Giuseppe Palladino, D'Amore Claudio, Patrizia Ricci, Eleonora Distrutti, Miriam Barbanti, Franco Baldelli, Stefano Fiorucci, Inhibition of NF-κB by a PXR-dependent pathway mediates counter-regulatory activities of rifaximin on innate immunity in intestinal epithelial cells. European Journal of Pharmacology. ,vol. 668, pp. 317- 324 ,(2011) , 10.1016/J.EJPHAR.2011.06.058
B. Truszkowska, E. Parmigiani, Treatment of endometritis in cows and mares; intrauterine infusion of antibiotic foam. Summa. ,vol. 7, pp. 43- 46 ,(1990)
François Denizot, Rita Lang, Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Journal of Immunological Methods. ,vol. 89, pp. 271- 277 ,(1986) , 10.1016/0022-1759(86)90368-6
Jenni Küblbeck, Vanessa Zancanella, Viktoria Prantner, Ferdinand Molnár, E. James Squires, Mauro Dacasto, Paavo Honkakoski, Mery Giantin, Characterization of ligand-dependent activation of bovine and pig constitutive androstane (CAR) and pregnane X receptors (PXR) with interspecies comparisons Xenobiotica. ,vol. 46, pp. 200- 210 ,(2016) , 10.3109/00498254.2015.1060374
M. A. Fortier, L. A. Guilbault, F. Grasso, Specific properties of epithelial and stromal cells from the endometrium of cows Reproduction. ,vol. 83, pp. 239- 248 ,(1988) , 10.1530/JRF.0.0830239
Jie Cheng, Yatrik M. Shah, Frank J. Gonzalez, Pregnane X receptor as a target for treatment of inflammatory bowel disorders Trends in Pharmacological Sciences. ,vol. 33, pp. 323- 330 ,(2012) , 10.1016/J.TIPS.2012.03.003
Andrea Mencarelli, Marco Migliorati, Miriam Barbanti, Sabrina Cipriani, Giuseppe Palladino, Eleonora Distrutti, Barbara Renga, Stefano Fiorucci, Pregnane-X-receptor mediates the anti-inflammatory activities of rifaximin on detoxification pathways in intestinal epithelial cells Biochemical Pharmacology. ,vol. 80, pp. 1700- 1707 ,(2010) , 10.1016/J.BCP.2010.08.022
Xiaochao Ma, Yatrik M. Shah, Grace L. Guo, Ting Wang, Kristopher W. Krausz, Jeffrey R. Idle, Frank J. Gonzalez, Rifaximin Is a Gut-Specific Human Pregnane X Receptor Activator Journal of Pharmacology and Experimental Therapeutics. ,vol. 322, pp. 391- 398 ,(2007) , 10.1124/JPET.107.121913
Shan Herath, Sonia T. Lilly, Deborah P. Fischer, Erin J. Williams, Hilary Dobson, Clare E. Bryant, I. Martin Sheldon, Bacterial Lipopolysaccharide Induces an Endocrine Switch from Prostaglandin F2α to Prostaglandin E2 in Bovine Endometrium Endocrinology. ,vol. 150, pp. 1912- 1920 ,(2009) , 10.1210/EN.2008-1379