Spinal bromodomain-containing protein 4 contributes to neuropathic pain induced by HIV glycoprotein 120 with morphine in rats

摘要: The symptoms of HIV-sensory neuropathy are dominated by neuropathic pain. Recent data show that repeated use opiates enhances the chronic pain states in HIV patients. Limited attention has so far been devoted to exploring exact pathogenesis painful disorder and opiate abuse vivo, for which there is no effective treatment. Bromodomain-containing protein 4 (Brd4) a member bromodomain extraterminal domain (BET) family functions as chromatin 'reader' binds acetylated lysines histones brain neurons mediate transcriptional regulation underlying learning memory. Here, we established model interaction intrathecal envelope glycoprotein 120 (gp120) morphine rats. combination gp120 (gp120/M, 5 days) induced persistent mechanical allodynia compared with either or alone. Mechanical reached lowest values at day 10 from gp120/M application, beginning recover 21. In model, overexpression Brd4 mRNA using RT-qPCR western blots, respectively. Immunohistochemical studies showed was expressed spinal cord dorsal horn. BET inhibitor I-BET762 dose-dependently increased threshold state. present study provides preclinical evidence treating opioids inhibitor.