Bacteriophage N4-Coded, Virion-Encapsulated DNA-Dependent RNA Polymerase

摘要: Publisher Summary This chapter reviews the bacteriophage N4 vRNAP and its unique ability to transcribe promoter-containing single-stranded DNAs with specificity, providing a tool for synthesis of RNAs from synthetic oligonucleotide templates or cloned in M13 DNA. It is an ideal enzyme short large amounts at limiting template concentrations presence Eco SSB, several reasons. displays high fidelity and, contrast T7 RNAP, does not appear produce abortive products. discusses length transcripts that can be synthesized by mini-vRNAP limited available DNA template. useful specific RNA–DNA hybrids when transcription carried out absence SSB. The explores discovery has provided study polymerase–promoter, polymerase–substrate, polymerase–product interactions. Finally, identification transcriptionally active domain raises questions on possible roles amino carboxy-terminal domains polypeptide. Experiments progress have indicated these virion morphogenesis, initial steps phage infection.