Enhancement of serum fibronectin adsorption and the clonal plating efficiencies of Swiss mouse 3T3 fibroblast and MM14 mouse myoblast cells on polymer substrates modified by radiofrequency plasma deposition

摘要: Abstract Surfaces which specifically enhance fibronectin (FN) adsorption from serum containing media were prepared and investigated. Since FN adsorbed to a substrate facilitates the spreading subsequent replication of anchorage-dependent cells in culture, these new surfaces interest. The studied by treating either poly(ethyleneterephthalate) (PET) or polystyrene (PS) with radiofrequency plasma discharge ethylene oxide (C2H4O) perfluoropropane (C3F8) vapor, mixture two, one following vapors: acetone, air, methanol, water. analyzed electron spectroscopy for chemical analysis (ESCA). Identification quantitation surface functional groups was done high resolution ESCA. series dilutions modified plastic measured using Iodine (125I)-labeled FN. Swiss mouse 3T3 fibroblasts MM14 skeletal myoblasts each plated onto surfaces, as well on two different types commercially available culture dishes. Plating efficiency surface. cell after 2 h, kinetics, attachment kinetics also some surfaces. All PET PS plasma-treated except those treated C3F8 more than did three maximal at intermediate concentration all greatest enhancement occurred acetone Bovine albumin less that untreated plating efficiencies both correlated but behavior commercial tissue dishes not always follow this trend. In general, exhibiting had greater fraction C-O functionalities low efficiency, substrates. possible role other factors mediating enhanced growth is discussed.