In-vivo topical mucosal delivery of a fluorescent deoxy-glucose delineates neoplasia from normal in a preclinical model of oral epithelial neoplasia.

摘要: Metabolic imaging of oral cavity mucosal surfaces could benefit early detection squamous cell carcinoma (OSCC) and epithelial dysplasia (OED). Fluorescent deoxy-glucose agents provide contrast for glucose metabolism similar to 18FDG-PET allow use optical imaging, which provides high resolution lower potential cost. However, in-vivo topical delivery fluorescent without injection or tissue resection has not been shown. We introduce neoplasia following 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose (2-NBDG) in an OSCC/OED hamster model demonstrate uptake into epithelium across the surface disrupting epithelium. 2-NBDG fluorescence intensity 30-minutes application was 6-fold 4-fold higher OSCC OED, respectively, compared normal mucosa. Receiver operator characteristic analysis show 83% sensitivity 73% specificity vs benign (normal inflammation). Faster temporal decay indicated metabolic rate than Mucosal by is feasible delineates from mucosa, providing noninvasive molecular dysregulated metabolism, preclinical studies carcinogenesis be developed detection.