Acute rejection of experimental lung allografts: characterization of intravascular mononuclear leukocytes.

摘要: Abstract Leukocytes interacting with endothelia of lung allografts probably play a seminal role in acute rejection, but have not been characterized before. Transplantation was performed the Lewis to and Dark Agouti rat strain combinations. DNA replication detected T-cells on day 2 after pulse-labelling vivo 5-bromo-2′-deoxyuridine (BrdU). On 5, leukocytes were isolated by intensive perfusion graft, subject flow cytometry quantitative RT–PCR. About 34 million accumulated allograft vessels, only 10 6 million cells isografts control lungs, respectively. During IFN-γ, IL-1β IL-10 mRNA expression increased, IL-12 decreased, whereas IL-2, IL-6, TNF-α, TGF-β did change. The phenotype graft monocytes partially activated intravascular proliferated. In conclusion, during unusual properties accumulate T-lymphocytes are blood vessels.