Anti-angiogenic effect of silymarin on colon cancer LoVo cell line.
作者： Shung-Haur Yang , Jen-Kou Lin , Wei-Shone Chen , Jen-Hwey Chiu
DOI: 10.1016/S0022-4804(03)00229-4
关键词:
摘要: Abstract Objective This study was designed to evaluate the anti-angiogenic effect of silymarin (SM) and its major pure component silibinin (SB), also thalidomide (TH). Materials methods A modified in vitro system using a coculture endothelial (EA.hy 926) colon cancer (LoVo) cell lines adopted this study. Results In cytotoxicity assay, SM/SB/TH concentrations causing 20% (IC 20 ) inhibition cellular growth were 41.8 μg/ml/0.22 mM/0.088 mM for EA.hy 926 cells, 16.1 μg/ml/0.12 mM/0.099 LoVo respectively. All 3 drugs showed concentration dependent migration differentiation assay. The IC 50 inhibiting chemotaxis towards by 1.15 μg/ml/0.66 μ m /1.98 , inhibited capillary tube formation 50% at 1.25 μg/ml/2.6 /6.3 an analysis vascular factor secreted decreased secretion 6.52 μg/ml/6.6 /131.7 Conclusion SM/SB has strong anti-angiogenesis on line, might provide alternative treatment option anti-cancer treatment.
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