Carbohydrate Moieties in Recombinant Human Thyroid Peroxidase: Role in Recognition by Antithyroid Peroxidase Antibodies in Hashimoto’s Thyroiditis

摘要: We studied the oligosaccharide moieties of recombinant human thyroid peroxidase (hTPO) expressed in Chinese hamster ovary (CHO) cells, and role these glycans hTPO antigenicity Hashimoto's thyroiditis. To determine whether carbohydrate were AT-linked, O-linked, or both, to obtain information about characteristics component(s), we digested with deglycosylating enzymes varying specificity. Proteins CHO-TPO cells labeled [35S]methionine, was immunoprecipitated anti-hTPO antibodies present thyroiditis serum. Digestion endoglycosidase (endo) F, which removes both complex polymannose iV-linked glycans, increased electrophoretic mobility doublet from approximately 115 kD 110 105 kD. Endo H, acts similarly endo but only on polymannose, not complex, had a similar effect. In contrast, O-glycanase neuraminidase, remove O-linked glycan...