Heritable gene silencing in Drosophila using double-stranded RNA.

摘要: RNA-mediated interference (RNAi) is a recently discovered method to determine gene function in number of organisms, including plants, nematodes, Drosophila, zebrafish, and mice. Injection double-stranded RNA (dsRNA) corresponding single into organisms silences expression the specific gene. Rapid degradation mRNA affected cells blocks expression. Despite promise RNAi as tool for functional genomics, injection dsRNA interferes with transiently not stably inherited. Consequently, use study late stages development has been limited. It particularly problematic disease models that reply on post-natal individuals. To circumvent this problem we have developed express an extended hairpin-loop RNA. This successful generating nematode Caenorhabditis elegans. The hairpin expressed from transgene exhibiting dyad symmetry controlled temporal spatial pattern. We report inherited confers embryos, tissues give rise adult structures such wings, legs, eyes, brain. Thus, can be adapted late-acting Drosophila. success approach Drosophila C. elegans suggests similar may prove useful higher which transgenic technology available.