Genotoxic effect and rat hepatocyte death occurred after oxidative stress induction and antioxidant gene downregulation caused by long term fluoride exposure.

摘要: Studies focusing on possible genotoxic effects of excess fluoride are contradictory and inconclusive. Currently, studies have reported a probable link to oxidative stress, DNA damage apoptosis induced by in rat hepatocytes. We developed an in vivo study administering three doses gavage given rats for 60 day. Micronucleus test was applied investigate potential fluoride. The TUNEL method determined fragmentation apoptosis. Biochemical parameters mitochondrial swelling stress. Semi-quantitative RT-PCR immunostaining determine mRNA protein expression antioxidant enzymes. Analyses the hepatic function morphology were performed. Our results revealed but did not confirm nor increase positive labelling fluoride, indicating absence Oxidative stress induction confirmed is probably associated damage. Cell death events such as empty nuclear spaces, cytoplasm degeneration, pyknosis, karyorrhexis followed karyolysis observed. Hepatic appear be significantly modified makes no evidence necrosis suggesting other cell pathway, autophagic. In conclusion, prolonged intake at chosen concentrations caused imbalance cellular state, affected disrupted homeostasis. It recommended that supplementation requires fresh consideration light current study.