Caveolin isoform switching as a molecular, structural, and metabolic regulator of microglia

摘要: Microglia are ramified cells that serve as central nervous system (CNS) guardians, capable of proliferation, migration, and generation inflammatory cytokines. In non-pathological states, these exhibit morphology with processes intermingling neurons astrocytes. Under pathological conditions, they acquire a rounded amoeboid proliferative migratory capabilities. Such morphological changes require cytoskeleton rearrangements. The molecular control points for polymerization states microtubules actin still under investigation. Caveolins (Cavs), membrane/lipid raft proteins, expressed in cells, yet the role caveolin isoforms microglia physiology is debatable. We propose caveolins provide necessary point regulation cytoskeletal dynamics, thus investigated biology. detected mRNA protein both Cav-1 Cav-3. was significantly less localized to plasmalemma (PM) cytoplasmic vesicles (CVs) microglial inactive state, while active (amoeboid-shaped) exhibited increased expression. contrast, Cav-3 highly state cellular perinuclear regions microglia. Pharmacological manipulation or non-active altered Additionally, expression also mitochondrial respiration, suggesting possible regulatory roles cell metabolism facilitate changes. present findings strongly suggest activity part due isoforms, providing promising novel therapeutic targets CNS injury disease.