Apparatus and methods for multianalyte homogeneous fluoroimmunoassays

摘要: Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The employs a planar waveguide (124) with an integral semi-cylindrical lens (502), has multi-analyte features calibration features, along improved field intensity. A preferred embodiment of the biosensor (120) assay method have patches (662) capture molecules (720) each specific different analyte (210), disposed adjacent within single reservoir (660). immobilized to on surface by site-specific coupling thiol groups photo-affinity crosslinkers (706), which in turn coupled (700) or non-specific-binding-resistant coating (702) (700). (662, 664, 666) antibodies produced selectively irradiating portion (714) during process selective irradiation involving mask (712), laser source, like.