System and methods for monitoring for dna amplification by fluorescence
作者： Carl T. Wittwer , Kirk M. Ririe , David R. Hillyard , Randy P. Rasmussen
DOI:
关键词:
摘要: A thermal cycling method and device is disclosed. The comprises a sample chamber whose temperature can be rapidly accurately modulated over range of temperatures needed to carry out number biological procedures, such as the DNA polymerase chain reaction. Biological samples are placed in glass micro capillary tubes then located inside chamber. programmable controller regulates Monitoring amplification monitored by fluorescence once per cycle or many times cycle. present invention provides that monitoring PCR powerful tool for quantification.
lens.org 参考文章(14)
Robert E. Fields, Molecular analyzer and method of use ,(1995)
Gerald L. Klein, Method for measuring specific binding assays ,(1994)
Paul M. Lizardi, Sanjay Tyagi, Fred R. Kramer, Hybridization probes for nucleic acid detection, universal stems, methods and kits ,(1994)
Hitoshi Tateoka, Kouichi Karaki, Masahiro Ohno, Akihiro Namba, Outaro Ando, Fumio Uchino, Tatsuo Nagasaki, Method and apparatus for measuring immunological reaction with the aid of fluctuation in intensity of scattered light ,(1988)
Sanjay Tyagi, Fred Russell Kramer, Molecular Beacons: Probes that Fluoresce upon Hybridization Nature Biotechnology. ,vol. 14, pp. 303- 308 ,(1996) , 10.1038/NBT0396-303
Paul R. Selvin, John Hearst, Luminescent lanthanide chelates and methods of use ,(1995)
Michael J. Heller, Edward J. Jablonski, Fluorescent stokes shift probes for polynucleotide hybridization assays ,(1986)
Vera Janatova, Shao-Chie Huang, Douglas A. Christensen, James N. Herron, Hsu-Kun Wang, Karin D. Caldwell, Apparatus and methods for multianalyte homogeneous fluoroimmunoassays ,(1994)
Kevin S. Bodner, Alan M. Ganz, John Shigeura, Charles R. Connell, Eugene F. Young, David H. Tracy, Timothy M Woudenberg, Linda G. Lee, Paul G. Saviano, Lincoln J. Mcbride, System for real time detection of nucleic acid amplification products ,(1995)