Transcriptional profiling of Arabidopsis root hairs and pollen defines an apical cell growth signature

摘要: Current views on the control of cell development are anchored notion that phenotypes defined by networks transcriptional activity. The large amounts information brought about transcriptomics should allow definition these through analysis cell-specific signatures. Here we test this principle applying an analogue to comparative anatomy at cellular level, searching for conserved signatures, or small gene-regulatory (GRNs) root hairs (RH) and pollen tubes (PT), two filamentous apical growing cells a striking example conservation structure function in plants. We developed new method isolation mature hair cells, analysed their transcriptome microarray analysis, further compared it with other single data. Principal component shows statistical relation between datasets RHs PTs which is suggestive common profile pattern plant, overlapping profiles clear similarities level GTPases, vesicle-mediated transport various specific metabolic responses. Furthermore, cis-regulatory element co-regulated genes revealed binding sequences likely required expression comprising signature. This included significant occurrence motifs associated response upon anaerobiosis. Our results suggest maintaining growth mechanisms synchronized energy yielding might require combinatorial network regulation. propose study constitute foundation genetic physiological dissection underlying plant cells.