Abraxas and RAP80 Form a BRCA1 Protein Complex Required for the DNA Damage Response
作者： B. Wang , S. Matsuoka , B. A. Ballif , D. Zhang , A. Smogorzewska
关键词:
摘要: The BRCT repeats of the breast and ovarian cancer predisposition protein BRCA1 are essential for tumor suppression. Phosphopeptide affinity proteomic analysis identified a protein, Abraxas, that directly binds through phospho-Ser-X-X-Phe motif. Abraxas to mutual exclusion BACH1 (BRCA1-associated C-terminal helicase) CtIP (CtBP-interacting protein), forming third type complex. recruits ubiquitin-interacting motif (UIM)–containing RAP80 BRCA1. Both were required DNA damage resistance, G2-M checkpoint control, repair. was optimal accumulation on damaged (foci) in response ionizing radiation, UIM domains alone capable foci formation. RAP80-Abraxas complex may help recruit sites part recognition ubiquitinated proteins.
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