Mitotic quiescence, but not unique “stemness,” marks the phenotype of bone metastasis-initiating cells in prostate cancer

摘要: This study aimed to identify subpopulations of prostate cancer cells that are responsible for the initiation bone metastases. Using rapidly dividing human cell lines, we identified mitotically quiescent (<1%), which compared with populations patterns gene expression and their ability migrate skeletons athymic mice. The used 2-photon microscopy map presence/distribution fluorescently labeled, luciferase determine presence growing We showed were very significantly more tumorigenic in forming metastases than fast-growing (55 vs. 15%) had a unique profile. not uniquely stem like, no CD133 but same level other putative markers (CD44 integrins α2/β1), when proliferating population. In addition, mitotic quiescence was associated high levels C-X-C chemokine receptor type 4 (CXCR4) production. Inhibition CXCR4 activity altered homing tumor bone. Our studies suggest dormancy is phenotype facilitates colonization skeleton cancer.