Targeting the rhesus macaque TRIM5α gene to enhance the susceptibility of CD4+ T cells to HIV-1 infection.

摘要: The host range of human immunodeficiency virus type 1 (HIV-1) is extremely narrow, which has hampered the establishment non-human primate models for HIV-1 infection. species-specific innate immune factor tripartite motif 5 alpha (TRIM5α) a key molecule that confers potent resistance against In this study, we targeted TRIM5α gene rhesus macaques (rhTRIM5α) using transcription activator-like effector nuclease (TALEN) to study effect on CD4+ T cells were separated from peripheral blood by magnetic cell sorting, and positive rate was greater than 99%. TALEN plasmids targeting rhTRIM5α constructed introduced into electroporation, with transfection efficiency approximately 25%. genome extracted, target analyzed T7E1 enzyme digestion. After sorting transductants, TALENs induced mutations at more 40%. ability infect demonstrated ELISA. results showed enhanced susceptibility This finding will pave way further new macaque model studies.